Combined effect of recombinant human bone morphogenetic protein-2 and low level laser irradiation on bisphosphonate-treated osteoblasts
정석영, Hong Ji-Un, 송재민, 김인령, 박봉수, 김철훈, 신상훈,
소속 상세정보
정석영 ( Jeong Seok-Young ) - Pusan National University School of Dentistry Department of Oral and Maxillofacial Surgery
( Hong Ji-Un ) - Pusan National University School of Dentistry Department of Oral and Maxillofacial Surgery
송재민 ( Song Jae-Min ) - Pusan National University School of Dentistry Department of Oral and Maxillofacial Surgery
김인령 ( Kim In-Ryoung ) - Pusan National University School of Dentistry Department of Oral Anatomy
박봉수 ( Park Bong-Soo ) - Pusan National University School of Dentistry Department of Oral Anatomy
김철훈 ( Kim Chul-Hoon ) - Dong-A University Hospital Department of Oral and Maxillofacial Surgery
신상훈 ( Shin Sang-Hun ) - Pusan National University School of Dentistry Department of Oral and Maxillofacial Surgery
Abstract
Objectives: The purpose of this study was to evaluate the synergic effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) and low-level laser therapy (LLLT) on bisphosphonate-treated osteoblasts.
Materials and Methods: Human fetal osteoblast cells (hFOB 1.19) were cultured with 100 μM alendronate. Low-level Ga-Al-As laser alone or with 100 ng/mL rhBMP-2 was then applied. Cell viability was measured with MTT assay. The expression levels of receptor activator of nuclear factor kappa-B ligand (RANKL), macrophage colony-stimulating factor (M-CSF), and osteoprotegerin (OPG) were analyzed for osteoblastic activity inducing osteoclastic activity. Collagen type and transforming growth factor beta-1 were also evaluated for bone matrix formation.
Results: The results showed that rhBMP-2 and LLLT had a synergic effect on alendronate-treated osteoblasts for enhancing osteoblastic activity and bone matrix formation. Between rhBMP-2 and LLLT, rhBMP-2 exhibited a greater effect, but did not show a significant difference.
Conclusion: rhBMP-2 and LLLT have synergic effects on bisphosphonate-treated osteoblasts through enhancement of osteoblastic activity and bone formation activity.
키워드
Alendronate; Osteoblasts; Bone morphogenetic protein 2; Low-level laser therapy
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